Which of the following methods is most suitable to determine

Which of the following methods is most suitable to determine if the actin cytoskeleton is dynamic and constantly undergoes assembly and disassembly? (1 point)

a. Immunofluorescence microscopy with a primary antibody to G-actin (raised in mouse) and a secondary anti-mouse antibody (raised in goats) coupled to a green fluorophore.

b. Live imaging of cells expressing GFP-tagged actin

c. FRAP analysis in cells expressing GFP-tagged actin

d. Electron microscopy

Solution

The correct answer is :b. Live imaging of cells expressing GFP-tagged actin

Reason:Since the actin cytoskeleton is not static and rearranges itself ,live cell imaging of cells that express GFP-tagged actin is the best way to determine dymanics of actin cytoskeleton.