This is a graded discussion: 3 points possible due Feb 13 at Since this is a group discussion each group has its own conversation for this topic.Here are the ones you have access to Discussion Board Question 2 Please answer the question below. Minimum participation standard: You must post one original response and one return comment to classmates. You will not be able to see other student\'s response until you answer the question. This discussion question addresses and aligns with the following Course Learning objectives: 1. Recognize microbiology laboratory methods and practices 2. Identify lab equipment usage and techniques involved in the microbiology laboratory 5. Recognize staining techniques, sterile techniques. culture preparation and growth. Addresses and aligns with Aseptic Technique Lab Learning Objective-Recall the importance of aseptic technique in transferring organisms and maintaining pure cultures Topic from Lab 4: Aseptic Techniques Imagine you need to inoculate a tryptone broth tube with E. coli When you examined the broth tube during the next lab period, there was no growth at all. What could have gone wrong with this experimen Unread Search entries or author Reply
Answer A. Above question: While performing inoculation loop was not cool adequately hot loop can kill bacteria so he inoculated dead bacteria in broth .The culture could possibly been having dead culture.
1.Answer : Laboratory of microbiology should follow fixed standard for microbiology lab for food test FDA has set standard for such check of microbes.Micobiological analytical manual is related with microbiology laboratory method.
2. Answer: Equipment is used in microbiology lab are :
3.Answer: Staining technique:
Make smear, air dry , heat fix, stain with crystal violet ,stain with iodine
,decolorize with alcohol, stain with sefranin and wash ,blot dry view under microscope.
Sterile technique inculde use of whole operation under syrole condition under laminar flow, use of gloves etc sterlize glasswares.
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