7 Which of the following sets of primers could you use to am

7. Which of the following set(s) of primers could you use to amplify the target DNA sequence indicated by the dots?

a. 5\' GGAAAATTCAGATCTTAG 3\' and 5\' TGGGCAATAATGTAGCG 3\' b. 5\' GCTAAGATCTGAATTTTC 3\' and 3\' ACCCGTTATTACATCGC 5\'

c. 3\' GATTCTAGACTTAAAGGC 5\' and 3\' ACCCGTTATTACATCGC 5\' d. 5\' GCTAAGATCTGAATTTTC 3\' and 5\' TGGGCAATAATGTAGCG 3\'

5\' GGCTAAGATCTGAATTTTCCGAG . . . TTGGGCAATAATGTAGCGCCTT 3’

3\' CCGATTCTAGACTTAAAAGGCTC . . . AACCCGTTATTACATCGCGGAA 5\'

Solution

option b.

5\' GCTAAGAT...3\' would bind at 3\' end (of lower sequence in the given double stranded DNA, leaving first nucleotide)

5\' CGCTACA...3\' would bind at 3\' end (of upper sequence in the given double stranded DNA, leaving first four nucleotides)