Give three basic components that make vectors useful for clo

Give three basic components that make vectors useful for cloning and describe the purpose for each.

Solution

Answer : The three most important components of a vectors includes

Seletable markers : Slectable markers such tetracycline resistance and amicillin resistant genes which helps the researchers to screen for the clones that harbors the gene of interest by growing the bacteria in the media that contains these antibiotics which allows the growth of only clones that has plasmid with these resistant genes.

Origin of replication/ori C sites : Which is required for the initiation of DNA replication in the host cells

Multiple cloning site/polylinker : It is a DNA sequence which contains many restriction sites. An MCS increases the number of potential cloning strategies available by extending the range of enzymes that can be used to generate a restriction fragment suitable for cloning.

Cloning vectors used for the generation of multiple copies of a particular gene. Cloning vectors consists of origin of replication, selectable marker such as ampicillin resistance genes and multiple cloning sites.

Examples of cloning vectors: plasmids (PBR322 and PUC18), lambda phage, BACs, YACs etc.,