1 you inoculate an LB tube with an aerobic bacterium and inc

1) you inoculate an LB tube with an aerobic bacterium and incubate it for 24 hours. What kind of growth do you expect to see and why?

2) True or False, \"you must use a fresh loop culture for each quadrant when performing the quadrant streak method.\"

Solution

Ans1 . when we inoculate an LB tube with an aerobic bacterium and incubate it for 24 hours we would observe that the liquid media becomes cloudy if bacteria are present. This could be the result of only one bacterial cell originally entering the medium then dividing repeatedly to produce millons and as result an isolated colony is formed.This is useful when we need to study the colony morphology of an organism.

Ans 2. It is true that we must use a fresh loop culture for each quadrant because we need to flame the loop before and after each use and these loops are used to transfer the microorganism and needs to be strelized