You set up the following gel in an attempt to construct a re

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases Ban ll and Bg/ll. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... What is the number of restriction sites of Bg/ll in rho UK1? 1 2 3 4 5

Solution

11. (A) 1

Lane 3 represents pUK1 digested with BgII enzyme. Since the plasmid is circular a single cut generates a linear plasmid. Hence we see just one band.