1 You have isolated a new nuclear protein whose expression l

1. You have isolated a new nuclear protein, whose expression level is signficantly increased in cancer. Careful sequence analysis reveals the following sequence in the N-terminus of this protein (the positions of the amino acids are indicated): Pro Tyr (12)-lle(13) Epi. A construct in which amino acids 0 are deleted does not localize to the nucleus. However, when you fuse amino acids Pro(2) Val (3)-Ala(4)-Arg(5) -Lys(6) -Lys(7)-Arg(8) Lys(9) Lys(10) Lys 0 to GFP, GFP remains in the cytoso Which of the following statements best describes the conclusion that you would draw from this experiment? amino acids 1-10 are necessary to direct protein import into the nucleu s 1-10 represents the DNA binding domain of this protein deletion of amino acids 1-10 disrupt the import of all proteins into the nucleus amino acids 1-10 are sufficient for the nuclear import of this protein amino acids 1-10 are commonly cleaved off by signal peptidase 2 in the protein from question 1, Arg in position 8 is mutated to Asp. Which of the following statements best describes the phenotype produced by this mutation? ll be misshaped because the nuclear lamina Cannot form transcription of various genes your novel protein will not be imported into the nucleus Ran-GTP will remain in the cytosol the import of all proteins into the nucleus will be blocked 3. Which of the following statements best describes a shared characteristic of co-translational protein import into the ER and protein import into the mitochondrial matrix? proteins to be imported are recognized by SRP Proteins to be imported are unfolded by a chaperone of the Hsp70 famil both organelles have to be in an Protein import depends on the activation state of a small GTPase Protein import depends on an ATPase that pulls the protein across the membrane 4. A screen has identified a mutant cell that is unable to migrate. Sequence analysis reveals the presence of two mutations in the transmembrane domain of the Integrin alpha 6, that localizes to the plasma membrane, and which plays a critical role in cell adhesion. The mutations are as follows: Leu Asp and Trp Lys Which of the following statements best describes the reason for why the mutant cell does migrate in a fibronectin-coated dish? The cell does not migrate because it cannot form desmosomes it cannot form an adhesion belt it cannot form the branched actin cytoskelton atthe leading edge annot adhere to the fibronectin in the dish t cannot link its microtubules to the extracellular matrix

Solution

Answer:-Option 1

Explanation :- amino acid 1_10 are necessary for nuclear transport of this protein because it acts as nuclear localization signal.