a conclusion for lab direct indirect Gram staining pleaseSol

a conclusion for lab direct ,indirect Gram staining please

Solution

In direct Gram staining, the basic dye used is positively charged, which combines with the bacterial membrane that is negatively charged. So that the organism appears stained.

In the indirect Gram staining method, acidic dye is used, which contains negatively charged portion. The negatively charged bacterial wall repel the dye. So that the background appears coloured and the organism appears colourless.