1 you mate two haploid strains of the following genotypes St

1. you mate two haploid strains of the following genotypes

Strain 1: HIS4 ura2                  Strain 2: his4 URA2

You mate the strains and sporulate the diploid. If the HIS4 and URA2 genes are 14 cM apart on the same chromosome, what percent of the resulting spores will be His- Ura-?

If you are having trouble with this question, try some practice problems in Linkage analysis.

2. A diploid with the following genotype is sporulated:

LEU2/leu2 TRP2/trp2

LEU2 is on ChrIII and TRP2 is on ChrV.

From your spore counts, you determine that you have 4 x 106 cells/ml. You dilute this culture and plate 100 l of the dilution onto YPD and onto SC-leucine media. By what factor should you dilute your culture to obtain about 100 colonies on SC-leucine?

Solution

1. The answer is c) 7%.

Due to recombination we can observe two different categories, the sum of two categories give the distance between two genes. As the distacne here is 14%, the probable percentage of His- Ura- is 14/2 = 7%.

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