In a transduction experiment the donor was c d e and the rec
In a transduction experiment, the donor was c^+ d^+ e^+ and the recipient was c d e. Selection was c^+. The four classes of transductants from this experiment are shown below. Determine the cotransduction frequency for c^+ and d^+. Determine the cotransduction frequency for c^+ and e^+. Which of the cotransduction frequencies represents the greater actual distance between genes?
Solution
5a cotransduction frequency for c+ and d+= number of cotransformed cells with c+ and d+ genotype /total number of transformed cells =76+57/500=133/500
=0.266=26.6%
5b cotransduction frquency for c+ and e+=number of cotransformed cells with c+ and e+ genotypes /total number of transformed cells=2+57/500=59/500=0.118=11.8%
5c cotransduction frequency is inversely propotional to the distance. Therefore cotransduction frequency for c+and e+ represents greater distance between the genes.
