Please explain conclusions 1 Given that the path length of t

Please explain!

conclusions 1. Given that the path length of the cuvette is 1 cm, what is the extinction coeficient of the 0020 mM Yellow 5 dye at lambda max? Words: 373

Solution

1. From the beer lambert\'s law

Molar extinction coefficient = absorbance / length of cuvette * concentration

= 440 / 1 x 0.02 = 22000 M^-1 cm^-1

2. Concentration of the diluted solution can be calculated using the formula

C1 x A1 = C2 x A2

0.075 x 1.84 = C2 x 0.78

C2 = 0.177 M