Suppose that a DNA closed circular duplex of 104 bps is a re

Suppose that a DNA closed circular duplex of 10^4 bp\'s is a relaxed structure containing B-form helix in normal aqueous solution. Compute the number and types of supercoils expected when the DNA is converted into the A-form helix by the addition of alcohol. Show the linking number, twist, writhe and super helical density. Suppose the DNA in pan a is relaxed in alcohol by the addition of a topoisomerase. The end result is a covalently closed relaxed circle in an alcohol-containing solution. When the enzyme is removed and the DNA is returned to normal aqueous buffer, what happens?

Solution

B) as soon as the relaxation enzyme topoisomerase is removed from the solution it will return back to its original form and shape..!!

A!) rotation per BP = 32.7.

Twist - +18\'

Diameter - 23A.

Turn per Helix - 28.6A