You have a protein of interest that constitutes about 02 of

You have a protein of interest that constitutes about 0.2% of the total protein in the starting material of E. coli extract and you purified it to nearly 98% using affinity chromatography How many folds of purification you could have achieved?

Solution

With Affinity chromatography you will attain 1000 fold purification. In the above question the protein is present in a relatively low content( only 0.2%) in the total protein . After purification by affinity chromatography which will result in more pure protein removing the other protein contaminants, the protein purified to nearly 98%. So the fold purification attained is approximatly 480 times.