A person becomes infected with Grossibacter symptomsii the c

A person becomes infected with Grossibacter symptomsii, the causative agent of disease. The person will start to have after symptoms after 30 generations. The bacteria will be growing exponentially for the entire duration of the infection with a growth rate of 2 generations/hr. How long will it take for symptoms to begin? Give the name of one method for measuring the number of bacteria in a sample and describe, in general, how the technique works. Finally, describe one limitation of this method of measurement.

Solution

Answer:

A)

30 generations: symptoms of disease started.

Growth rate is 2 generations for one hour means,

                          one generation = 30 minutes

                          30 generations = 15 hours

                    It takes 15 hours to begin symptoms

B)

Bacteria can count by total viable count method (TVC), in this method, bacterial inoculum is diluted in steril water and plated on petriplate containing the nutrient medium and observed for colony formation after 24 hours, and colonies are count as colony forming units (CFU).

In this method, range of colonies grown on petriplate should not be more than 200 CFU.