The Juno protein is made only in developing egg cells Assumi

The Juno protein is made only in developing egg cells. Assuming you have access to the mouse genome sequence, and assuming there is a single enhancer for the Juno gene, propose an experiment to determine the precise location of the enhancer associated with the Juno gene. Once you have found the position of the enhancer, you wish to determine what transcription factor(s) bind (s) to that enhancer In which cell type(s) would you try to find the transcription factor(s)? Why?

Solution

In order to find the location of the enhancer we will follow the following steps.

1.The first step is to check if an enhancer might be located upstream or downstream of the gene which it normally regulates.

2.The second step is to check if it is located in the several hundred thousand base pairs upstream or downstream of the start site.

3.The next step is to check within introns as enhancers can also be found within introns.

4.The next step is to check the chromosome as an enhancer may be inserted elsewhere in the chromosome, and still affect gene transcription

5.Finally we should check the exonic regdion as .enhancers can also be found at the exonic region of an unrelated gene.

The transcription factors binding to enhancer are:

1.CCCTC-binding factor or the CTCF which is the Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF genes.

Transcription factors are found in DNA(deoxy-ribobnucleic acid)AND RNA(ribonucleic acid) of cells because the transcriptionn factors bind the content contained in the DNA so the evilution can continue and the june gene can move ahead as well.