2 As we just discussed regulation er glucose lewehk in the b

2. As we just discussed, regulation er glucose lewehk in the blood depend on the signaling cascades kicked am by receptar binding of insulin gucagan and cascade that initiated by epinephrine, what happe to is here more, less, same, or depends and why 8, The Go subunit was Gai? You a active Glyoogen phosphoryase? c You had a phospho resistant Glycogen synthase? d. You had a catalytically dead mutation in PKA? e, You had a constitutively active phosphorylase kinase and a phosphomimetic (as if it is permanently phosphory ated) glycogen synthase Rank these situations from least glucose in blood to most gucose in blaod

Solution

a. Ga subunit converted to Gas means the active form of Ga and active glycolysis means less blood glucose.

b. If Glycogen phosphorylase is constitutively expressed then glucose 1-phosphate will always be there for glycolysis to perform and will be converted to glucose-6-Phosphate and glucose-6- phosphate does feedback inhibition of hexokinase( enzyme to convert glucose to glucose-6-phosphate) thus the accumulation of glucose will occur and blood glucose level will increase.

c.Phospho resistant glycogen synthetase will not be active then glucose 1 phosphate will not be converted to glycogen and will increase the blood glucose level by inhibiting the glycolysis.

d. PKA inactive enzyme will lead to the reduce cAMP concentration and glycogen phosphorylase will not get activated thus reducing the Glycolysis and increase in the blood glucose level.

e. Constitutive activation of phosphoryl kinase and the permanent phosphorylation of glycogen synthetase will stop the glycolysis and increase in the blood glucose level.