The restriction enzyme MalI cuts the doublestranded sequence

The restriction enzyme MalI cuts the double-stranded sequence 5-GATC-3 and its complement 3-CTAG-5 in the center between A and T to form blunt ends. For the following double-stranded sequence (note: the two strands\' sequences are separated by a slash), how many times does MalI cut the sequence, and how many fragments result after treatment with MalI?
5-GCACCGATCTGTGTTGTTCACCTAGCCCACGGTGGATCCAAGGC-3 / 5-GCCTTGGATCCACCGTGGGCTAGGTGAACAACACAGATCGGTGC-3

Solution

5-GCACCGATCTGTGTTGTTCACCTAGCCCACGGTGGATCCAAGGC-3

3\'CGTGGCTAGACACAACAAGTGGATCGGGTGCCACCTAGGTTCCG 5\'

Mal1 cuts the sequence at two sites resulting in 3 fragments. The 3 fragments are 9bp, 10bp and 25bp.

The restriction enzyme MalI cuts the double-stranded sequence 5-GATC-3 and its complement 3-CTAG-5 in the center between A and T to form blunt ends. For the fol

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