The restriction enzyme MalI cuts the doublestranded sequence
The restriction enzyme MalI cuts the double-stranded sequence 5-GATC-3 and its complement 3-CTAG-5 in the center between A and T to form blunt ends. For the following double-stranded sequence (note: the two strands\' sequences are separated by a slash), how many times does MalI cut the sequence, and how many fragments result after treatment with MalI?
5-GCACCGATCTGTGTTGTTCACCTAGCCCACGGTGGATCCAAGGC-3 / 5-GCCTTGGATCCACCGTGGGCTAGGTGAACAACACAGATCGGTGC-3
Solution
5-GCACCGATCTGTGTTGTTCACCTAGCCCACGGTGGATCCAAGGC-3
3\'CGTGGCTAGACACAACAAGTGGATCGGGTGCCACCTAGGTTCCG 5\'
Mal1 cuts the sequence at two sites resulting in 3 fragments. The 3 fragments are 9bp, 10bp and 25bp.
