A single chain pentapeptide of unknown sequence is reacted w

A single chain pentapeptide of unknown sequence is reacted with dansyl chloride. After acid hydrolysis and thin layer chromatography two fluorescent spots are observed. Which of the following amino acids may have reacted with dansyl chloride?

A) Glutamic acid

B) Methionine

C) Cysteine

D) Lysine

E) Histidine

Solution

A single chain pentapeptide is reacted with dansyl chloride. The polypeptide chain was subjected to thin layer chromatography, resulted in two fluorescent spots. The following amino acids may have reacted with dansyl chloride

N-terminal Amino acid and

D) Lysine,

E) Histidine