How can replication be increased in the Artemia bioassay to

How can replication be increased in the Artemia bioassay to improve reliability of results?

Solution

Answer: The replication be increased in the Artemia (brine shromp) bioassay to improve reliability of results using the Box-Behnken Design (BBD). In this design, the combined photoperiod and temperature have meticulous effects on the hatching percentage of Artemia cysts so that reliability of results also varies with hatching rate of Artemia cysts. The replication treatments include mainly 3 factors such as salinity, adequate temperature & photoperiod & these are treated equally with differential spaced treatments in different combinations. Therefore, replicated events are going to occur in healthy cells, which take tryptan blue cells finally, it is easier to differentiate between the \"dead and healthy brine shrimps\".