Prepare a bacteria suspension of 10 times 103 cellml from an

Prepare a bacteria suspension of 1.0 times 10^3 cell/ml from an original suspension contain 1.0 times 10^7 cell/ml (use serial dilution technique). Show all of the dilution steps, specify the volume and dilution factor of each tube.

Solution

Tube 1 contains 4.5 mL of sterile media; you will add 0.5 mL of the undiluted bacterial suspension to yield a total volume of 5.0 mL.

0.5mL 0.5mL                          1                      1:10 dilution

4.5mL + 0.5mL                      5.0mL                          10                      (1 x 10 ^-1)

Tube 2 contains 4.5 mL of sterile media; you will add 0.5 mL of the 1:10 diluted bacterial suspension to yield a total volume of 5.0 mL

0.5mL 0.5mL                          1 1 1:100 dilution

4.5mL + 0.5mL                      5.0mL                          10 100 (1 x 10 ^-2)

Tube 3 contains 4.5 mL of sterile media; you will add 0.5 mL of the 1:100 diluted bacterial suspension to yield a total volume of 5.0 mL

0.5mL 0.5mL                          1 1 1 1:1000 dilution

4.5mL + 0.5mL                      5.0mL                          10 100 1000 (1 x 10 ^-3)