which of the following techniques does not measure changes i

which of the following techniques does not measure changes in gene expression? western blot, southern blot, northern blot. explain your answer

Solution

Correct answer: Southern blotting is not used for checking gene expression.

Explanation: The Southern blot is used to detect the presence of a particular DNA fragment in a sample. It gives result about the DNA size and identity. It is not dealing with the protein or mRNA which are directly associated with gene expression.

Northern blot is a method used to detect specific RNA molecules in a mixture of RNA. It can be used to analyze a sample of RNA in order to measure the RNA expression of particular genes.

Western blot is used to detect and identify specific proteins in a sample.

Suppose a gene become mutated. Then its effect can be seen in the mRNA or in the protein. So, its expression can be detected by two processes. Either measuring the transcribed mRNA or analyzing the translated protein.

So, we can say that gene expression can be checked by Northern Blotting or Western Blotting.