Mutation 1 CG mutated to TA Mutation 2 AT mutated to GC Muta

Mutation 1 CG mutated to TA Mutation 2 AT mutated to GC Mutation 3 TA mutated to CG Which mutation (s) can be identified by using primer P? Mutation 1 Mutation 2 Mutation 3 More than one of the above

Solution

B. Mutation 2

Mutation 1 will not be identified as DNA polymerase will use the primer as template and initiate synthesis of the subsequent DNA.

Mutation 2 will be identified. During replication, at mutation site 2, C will be present which will allow incorporation of G in the sequence. Hence, mutation will be identified.

Mutation 3 will be not identified as mutation is outside the scope of replication i.e. after the binding of Primer Q.