Can someone explain this figure to me I have no idea what it

Can someone explain this figure to me? I have no idea what it\'s supposed to be expressing. The title of the article is \"Insulin Inhibits Hepatocellular Glucose Production by UtilizingLiver-enriched Transcriptional Inhibitory Protein to Disrupt theAssociation of CREB-binding Protein and RNA Polymerase II withthe Phosphoenolpyruvate Carboxykinase Gene Promoter\"

12 RLU CD wild GAL4- GAL4 type CEBPA DBD L L PEPCK-LUC C/U-PEPCK-LUC LUC LUC 30 RLU CBP 20 10 CD GAL4. wild GAL4 GAL4 type. CIEBPB DBD CBP CIU-PEPCK-LUC PEPCK-LUC LUC UA LUC FIG. 3. The accessory factor CIEBPB mediates hormone re- sponses and recruits CBP to potentiate PEPCK gene transcrip- tion. A, HATIE hepatoma cells were transiently transfected with either a wild-type PEPCK gene promoter-reporter construct (PEPCK-LUC) or a mutant construct in which the CRE is replaced by an upstream activating sequence (C/U-PEPCK-LUC) to which cotransfected, chi meric GAL4 proteins bind. After transfection, the cells were treated with the indicated hormones for 16 h and then harvested for luciferase assays. The bars depict the mean S.E. of at least three independent experiments. B, left, same as above, with the addition of cotransfected CBP. For direct comparison with results in A, the horizontal line inside each bar denotes the reporter strength exhibited in the absence of exogenous CBP. Right, GALA-CBP was cotransfected with the mutant C/U-PEPCK-LUC construct.

Solution

PEPCK is phosphoenolpyruvate carboxykinase.

The association of CREB binding protein or CBP and RNA polymerase with hepatic phosphoenolpyruvate carboxykinase, PEPCK gene promoter is enhanced by glucocorticoids and is disrupted by insulin.

Transcription can be speeded up through factors such as CCAAT/enhancer binding protein (C/EBP beta) which can recruit CBP.