Sample Preparation There are multiple ways that tissue speci

Sample Preparation: There are multiple ways that tissue specimens can be prepared for electron microscopy. Your book has some samples of techniques for varying types of samples. However, we would like to look at protocols (the method of completing the process from acquisition to polymerization) for a few different tissue samples. Your assignment is the following: create protocols for the following tissue: spleen, Liver, Kidney skeletal muscle, skin (a sectional representation containing both cutaneious and subcutaneous tissue), and cartilage. la .hinL Annnnnriato hnwever in creating these

Solution

Skin biopsy by wedge biopsy technique. This technique is usually performed for large lesions where not only length and breadth but also depth of specimen is important. Hence, this technique is commonly chosen for subcutaneous mycosis, margin of squamous cell carcinoma, tuberculosis verrucosa cutis, etc.

Protocol - After anaesthetizing the skin, a stab incision in a V or a triangular shape is performed with scalpel blade 11 or 21–25 so as to extract a cone of tissue. Most often the lesion is left to heal by secondary intention.