Please help Thanks Your measured absorbance in a quartz cuve

Please help. Thanks!

Your measured absorbance in a quartz cuvette with a 1 cm pathlength at 489 nm is 0.023. Using the Beer-Lambert Law, what is the concentration in molarity? In mg/mL? (See below for the extinction coefficient, .)

You graph your Bradford standards and fit a trendline with the equation y = 0.25x + 0.3. Your measured sample yields an absorbance of 0.11. What is the concentration of your sample (in mg/mL)?

Solution

In this question we don\'t have the value for molar extinction coefficient , so there is a formula to find out :-~ concentration = slope/absorbance

from the above equation :-

y=0.25+0.3

which is equals to y=mx+c

(where m is the slope and x is the concentration ,)

now , concentration = 0.25/0.023 = 10.86 mg/ml

- and same for the second case ,

concentration = slope/absorbance

concentration = 0.25/0.11 = 2.27mg/ml