Absorbance 420 nm Protein mix213 Fraction 10746 Fraction 202

Absorbance 420 nm:

Protein mix=2.13

Fraction 1=0.746

Fraction 2=0.285

Fraction 3= 0.147

Absorbance 280 nm:

Protein mix=0.727

Fraction 1=0.395

Fraction 2=0.385

Fraction 3= 0.341

Abs Abs Tota yield Fold Specific activity 420 600 280 protein (mg) For alkaline phosphatase purification (umol/min/mg) Protein mix Fraction 1 o 7 og 315 Fraction 2 0385 Fraction 3 NT o. Assume the extension coefficient of all these protein at 280 nm is 1 mg/mL/cm. Assume alkaline phosphatase\'s product has an extension coefficient of 0.1 umol/mL/cm. Which fraction has the most of enzyme?

Solution

Answer:

From the presented data, it is missing that how much time the test was conducted. Its looking fraction 1 is containing more content and hence probability of maximum presence of enzyme.