What would be the exact Materials and Method for this experi

What would be the exact Materials and Method for this experiment?

2. Material and methods
2.1. Subjects
One hundred obese children (58 girls and 42 boys) were studied
with a mean age (±SD) of 11.4 ± 2.7 (range 5–17 y). Obesity was de-
fined as a body mass index (BMI) above the 97th percentile for age
and sex according to the relevant national curves [28]. The participants
were recruited as part of the pediatric medical consultations of the Pre-
vention and Management Pediatric Obesity Network of Besançon
(France).
The study was conducted in accordance with the Declaration of Hel-
sinki and approved by the local ethics committee (Comité de Protection
des Personnes, Est-II, France, n°2015-A00763-46). All participants and
their parents or legal guardians were fully informed of the experimental
procedures and gave written informed consent.

2.2. Experimental procedures and measurements
After a medical exam performed by a pediatrician to ensure that the
participant had the ability to complete the whole protocol, the included
subjects underwent different assessments on the same day: i) anthro-
pometric characteristics and maturation status; ii) blood sample and
blood pressure; iii) physical activity level; iv) sleep-disordered
breathing.

Solution

2. Material and methods:

Assessment of anthro-pometric characteristics and maturation status in childhood who have obesity followed by examination of regular blood sample and monitoring blood pressure; monitoring of physical activity level as per regular ime scale, finally asking them for any presence of sleep-disordered breathing

2.1. Subjects - obese children --> One hundred obese children (58 girls and 42 boys) were studied with a mean age (±SD) of 11.4 ± 2.7 (range 5–17 y). Obesity was de-fined as a body mass index (BMI) above the 97th percentile for age and sex according to the relevant national curves