The following DNA sequences represent the first half of one

The following DNA sequences represent the first half of one strand of a restriction endonuclease recognition site. Using these sequences, write out the entire recognition site including both strands with 5\'- and 3\'- ends properly labeled. Additionally, search http://www.neb.com and identify each of the enzymes that would cleave these sites. Once the enzymes are identified, mark die position of enzyme cleavage on both strands. 5\'- GCA 5\'- CAT 5\'- AAG 5\'- GCGG

Solution

Enzymes for with sequence

1) 5\' GCA

2) 5\' CAT

3) GGCGGA - Ecil

enzyme clevage site is marked by /

GCAATG(2/0)	BsrDI
GCAATG	Nb.BsrDI
GCAGC(8/12)	BbvI
GCAGTG(2/0)	BtsI BtsI
GCAGTG	Nb.BtsI
GCANNNN/NTGC	BstAPI
GCATC(5/9)	SfaNI