make a 1L 10x stock of PBS Final concentration of reagents a

make a 1L 10x stock of PBS. Final concentration of reagents are 150mM NaCl, 20mM KCl, 11.5mM Na2HPO4, 8.5mM NAH2PO4.

Solution

The following are the steps to make 10x stock PBS:

1.The first step is to a dd each of reagents to 800 mL of pure water.

2.The next step is to adjust which can be done by adding enough pure water to make 1000mL of solution.

3.we need to make sure that the pH should be in between 7.2 and 7.6 and neither less nor more.

4.Lastly we need to autoclave and also make sure we can store at Room Temperature