Why must the spore stain include a heating step What would h

Why must the spore stain include a heating step? What would happen if this was omitted?

Why is staining bacterial components useful in strain identification?

Solution

Staining of a spore include heating step beacuse heat breaks the cell membrane (vegetative cell structure) and allows the stain (malachite etc) to bind with the spore. It means heat permeates the sopre for proper washing and binding of statin for the visualization of spore under microscope. If heat step is ommited then spore will not be permeable for the stain and the spore will not properly stained.

staining of bacterial components are neccassary for the its starin idendification.generally stains (dyes) are used which are organic compounds with two functional groups which gives different colors after binding with a particular substance present in the specimen. Cationic or basic dye (Crystal violet, Methylene blue safranin) will react with negative charge groups and anionic or acidic dye s (eosin and acid fuchsin) will bind to the positive charge groups.For bacterial strains identification Gram stain and acidfast stains are being used. Gram stains will identify wheather a bacteria has outer cell wall (gram negative) or they do not have (gram positive). Acid fast staining is also a differential stain used to identify acid fast organism. it includes Ziehl-Neelsen stain. It detects the mycoplasma containg mycolic acid.