Lab Subculting 3T3 Fibroblast Instructions make your own med

Lab: Subculting 3T3 Fibroblast

Instructions:

make your own media (10% FBS, 1% P/S, in DMEM),

be given a confluent plate of mouse 3T3 fibroblasts, and

subculture that plate using a 1:5 splitting ratio.

QUESTION:

Determine possible experiments you can run to test different media or substrates. Specifically, you will want to test their effect on cell proliferation, so:

imagine how you may set up this experiment, propose how you will determine whether an effect occurred or not? [provide a short protocol or step by step instructions]

Solution

In order to check effect of different media on 3T3 fibroblast following step one can follow