A culture of yeast was grown in a media containing sucrose l

A culture of yeast was grown in a media containing sucrose labeled by 14C at 2nd position of fructose. Determine the localization of the label in palmitic acid synthesized by the yeast.

Solution

GLYCOLYSIS result in synthesis of acetyl CoA

acetyl CoA formed from fructose will be labeled by 14C as 2nd position of fructose is labeled by 14C

this will result in sub cellular localisation of pamitic acid synthesis......by acetyl CoA which is labeled by 14C