Do you predict that bacterial transformation efficiency will

Do you predict that bacterial transformation efficiency will increase or decrease if you forget to add CaC12 to the cells? In one complete sentence, explain your choice I was thinking decrease but idk why

Solution

Yes,Bacterial Transformation efficiency will decrease beacuse CaCl2 provide the positive charge which promote the attaraction in between negatively charged Plasmid DNA and  lipopolysaccharides(present in Outer membrane of Bacterial cell wall).After short heat shock Plasmid DNA easily enter inside the Bacteria easily.