After which of the five treatments listed below can the plas

After which of the five treatments listed below can the plasmid shown simply by treating it with DNA lipase? Assume that after treatment any small pieces Q10-22 tom into a cinder pieces of DNA are removed, and it is the larger portion of plasmid that will reassemble into a circle.

Solution

Answer 1.

Cutting DNA with EcoR1 produces a sticky end. When you add the polymerase, it polymerizes new nucleotides to the sticky end, so that DNA fragment can be completed.

Note that polymerase adds nucleotides to the 3\' end of DNA; so, it in the cut G/CTTAA; DNA polymerase will add, AATTC after \'G\'. Please note that to the \'C\' in the end, \'G\' of HaeIII will form a hydrogen bond easily, and polymerization process will be accurate.