A LINEAR DNA fragment is cut with following enzymes and thei

A LINEAR DNA fragment is cut with following enzymes and their combinations. How many DNA fragments are expected from each digest EcoR1 BamH1 Pst1 EcoR1 + BamH1 BamH1 + Pst1 + EcoR1

Solution

The restriction enzymes that make staggered cuts leave small pieces of single-stranded DNA. Each restriction enzyme recognizes and can attach to a certain sequence on DNA called a restriction site. The enzymes that make staggered cuts leave small pieces of single-stranded DNA at the ends of the fragments they cut. At the end, totally 8 small DNA fragments are obtained from linear DNA.