Describe the relationship percent acrylamide and pore size o

Describe the relationship percent acrylamide and pore size of PAGE gets, give approximate quantitative values and typical dimension of a protein.

Solution

The pore size of polyacrylamide gels is attained by changing the Tand C values. ( T- total percentage concentration of acrylamide in the gel C- percentage of total monomer represented by crosslinker)

By altering the concentration of monomer in the gel the pore size can be altered in a reproducible manner the smaller the size of the greater the resolution.

So the port size of the gel is determine by two factors as mentioned it and see 4 size decreases with increasing p with cross linking 5% she gives the smallest pore size. any increase or decrease in percent from 5% increases the pore size.

Proteins are fixed by acetic acid and stained they are detected as blue bands on the background silver staining is used to detect trace amounts of proteins another staining method widely used is Coomassie brilliant blue staining. But silver staining is more accurate than coomassi brilliant blue staining.

The lowest level of detection of the proteins was approximately 0.21 ng from a total of 50 micrograms of the loaded protein. 2D electrophoresis in conjunction with silver staining 10 letters identify more than 300 proteins in 0.8 microlitre of Synovial fluid.