Mesendoderm read this otr2 Route rt works gatas sox32 gata6

Mesendoderm read this otr2 Route rt works gatas sox32 gata6 og9x Sox 17 foxa3 fora2 prdm1 gbx2 gata tbr16 ir.Ba sor4 trh.3 a) Describe an experiment how you could show that otr2 acts upstream from gata 5 and gata 6. Explain your reasoning. b) hich effect would a gain-of-function of gata 6 have on otx2 and gata 5 expression? Explain your reasoning. How would you generate gain-of- function of gata 6 in embryos? c) What effect would a loss-of-function of gata 5 have on gata 6 and sox 32? Explain your reasoning. How would you generate loss-of-function of gata 6 in embryos? d) How could you show that otx2 acts through gata 5 and gata 6 to turn sox 32 on Explain your reasoning

Solution

Ans. a) The gata5 and gata6 are essential for the specification of anterior haemangioblast . Interaction between these transcription factors is thoroughly analyzed and it is found that otx2 can activate both by acting upstream. In addition, it was also observed that the Gata family specification regulate the gata5 and gata6 to control the mesendoderm formation. Observations of chromatin immunoprecipitation are used to authenticate the conscription of Otx2 to the gata5 and gata6 loci.

b.) Gain-of-function experiments with these Gata factors describes the induction of cell spreading and migration in non-motile cells of ectoderm, whereas expression of a dominant-negative form of Gata6 severely hinders the ability of the dorsal leading edge of the mesendoderm to spread. Gain of function of gata5 in embryo generated by downstream action which provides the embryonic germ layers to gain ability to discriminate into distinct cell types. Gata5 also controls the amount of endoderm produced and promotes the expression of sox17 and foxa2.

c.) GATA6 is believed to have vital role in the development of several organs including the liver, gastrointestinal tract and heart. The conditional loss of function of gata6 resulted in a developmental arrest during gastrulation due to a requirement for these factors in the extra-embryonic endoderm.

d.) The gata 5 and gata 6 are basically transcription factor, essential for the development of endoderm during embryogenesis and also responsible for the regeneration of the differentiated epithelium in the mature gut.