Youve run an interrupted mating conjugation experiment with

You’ve run an interrupted mating conjugation experiment with Hfr cells that are hmm+ omg+ poo+ and streptomycin resistant (str^R). Your recipient cell is F- hmm- omg- poo- and streptomycin susceptible (str⁵). Below are the results from that interrupted mating experiment.

1) If you attempted to grow your exconjugants (those recipient cells that underwent conjugant) at 10 minutes on a 0 complete media plate that contained streptomycin, would you expect to see growth?

2) If you attempted to grow you exconjugants at 20 minutes on minimal media, what other supplement would you have to add to that minimal media in order to see growth?

3) Based on these data, draw a chromosomal map of you Hfr donor cell. Make sure you include in your drawing where the origin was located and clearly indicate somehow the direction of gene transfer.

frequency of Hfr genetic characters among str-resistant exconjugants 120 100 He 0102030 40 50 time (minutes) +0mg+--strR +hmm+-p00+

Solution

1. we dont seen the growth of species, if the exconjugates are formed biologically or accroding to media.(media componenet or cell growth accelerator is important consideration)

2. Minimal media are those which possed minimum amounnt of nutrition which is responsible for the growth, multiplication and cell colony formation it is oftenly used to produce wild strian of microbes. minimal media is extremely used for the developmnet of exconjugates,

it should contain

water,

various salts like magnesium, nitrogen, phosphusrus, sulphur for the protein synthesis,

it is containinnf some amino acids for the faster development

a carbon source, which may be a sugar such as glucose, or a less energy-rich source such as succinate.

3. based on above data it can not posssible to draw accurate chromosomal map