I need help with these questions Please explain the reasonin

I need help with these questions. Please explain the reasoning and how do you get the answer. Thank you

16. From the data in the question above, it is a. G. b. L. c. N d. can\'t be determined obvious that the Nterminal amino acid of the 1,382 fragment is: Subunit mass (Da) Native Protein mass (Da)point (pl) 17. Using the table to the right, what is the quaternary structure of protein B? a.. b. 8a. c.4B. d. 3x4y. e. 15a120 40,000 120,000 20,000 8.1 5.3 7.2 4.5 60.000 6.2 10,000 : 15,000 18. Which protein would migrate the fastest in gel-filtration chromatography? 20,000 25,000 75,000 30,000

Solution

18. B with 120,000 Da native mass

During gel filtration chromatography, the molecules are elated on the basis of their size. The larger molecules, do not get trapped in the beads of the gel, thus as a result they are elated first. Always while considering the elation, native mass of the protein is considered unless specified for a particular subunit.

17. 8a

The subunit size is given as 15000 Da, thus the tertiary structure will just contain the subunit, unless any other subunit is specified. When a 15000 Da will be repeated 8 times it will form a 120,000 Da proteins, thus giving a direct correlation. The other options are eliminated because they have more than one subunit in the options and they are not relevant.

16. For this question kindly provide more details.