You set up the following gel in an attempt to construct a re

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases BanII and Bg/II. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... Using the following bp sizes for the lambda fragments as a rough guide, what is estimated total size of the pUK1 plasmid? less than 3202bp 3202-5kb 7-9.25kb 9.05kb

Solution

For this answer you should know the size of fragments of lambda phage; which is not given. Now, since the question says, \"use the bp sizes for lambda fragments as a rough guide\" so I am answering it approximately.

According to research studies, Lamda phage has a linear DNA, 48502 bp long. When cut with bgl II, it gives the following fragments: 415, 22425, 35711, 38103, 38754, and 38814. So, the lowest fragement of lane I is 415kb followed by 22425, 35711, 38103, 38754, and 38814 bp respectively.

Lane 3 shows uncut pUK; this will give the size of pUK DNA. The size of pUK is between 38103 and 38754 approximately.

So, according to your options, A, ,B and C are totally ruled out. The correct answer is option \"D\" i.e. the size should be greater than 9050bp. Please note that the size is about 38.6kb