Match the specific cloning vectors with their features in ad

Match the specific cloning vectors with their features in a-d. Shuttle vectors________ Bacteriophage vectors_______ Cosmids_______ Artificial Chromosomes_______ a. A series of vectors containing lance pieces of DNA that can be used lo sequence an entire genome. b. Contains origins of replication for two organisms and can be transferred back and forth between them. C. Viral genomes with many genes removed so that foreign DNA can be inserted packaged. And transferred to E. coll. d. Vector containing only viral cos sites plus foreign DNA. Match the following protein to its function (a-c) during replication. DNA polymerase ______ DNA ligase ______ DNA gyraso ______ Single-stranded binding protein ______ Primase ______ a. Keeps the two complementary strands of DNA separated. b. Untwists strands of DNA. c. Links the 3\'-OH to the 5\'-PO_4 forming the phosphodiester bridge. d. Processively adds a complementary nucleotide to the 3\'end of a new strand of DNA. e. RNA polymerase used to synthesize short RNA primers. With respect to replication, match the following terms to its description. Origin of replication _______ Okazaki fragments _______ Semi-conservative ________ Replisome _______ Telomeres ________ a. Specific AT-rich sequence where replication begins b. Special structures found at the end of eukaryotic chromosomes c. Each new molecule consists of one completely new strand and one completely old s\' d. Small pieces of DNA on the lagging strand of DNA. e. Overall complex of proteins involved in replication.

Solution

Answer :

Q56:

Polylinker: Region of DNA with many restriction enzyme sites

Copy number : is how many molecules of DNA found in the cell

Antibiotic resistance gene: Means to select for the cells that received the vector

Alpha complementation: method to determine if foreign DNA has been inserted into the lacZ gene

Q57:

Shuttle vectors: viral genomes with many genes removed so that foreign DNA can be inserted packaged

Bacteriophage vectors: A series of vectors containing large pieces of DNA that can be used to sequence an entire genome

Cosmids: vectors containing only viral cos sites plus foreign DNA

Artificial chromosomes: Contains origin of replication for two organisms and can be transferred back and forth

Q 58:

DNA polymerase: Processively adds a complementary nucleotide to 3’ end of a new strand of DNA

DNA ligase : Links the 3’-Oh to the 5’-Po4 forming the phosphodiester bridge

DNA gyrase : Untwists strands of DNA

Single stranded binding protein: Keeps the two complementary strands of DNA separated

Primase: RNA polymerase used to synthesize short RNA primers

Q59:

Origin of replication: Specific AT rich sequence where replication begins

Okazaki fragments: Small pieces of DNA on the lagging strand of DNA

Semi-conservative: Each new molecule consists of one completely new strand and one completely old strand

Replisome: Overall complex of proteins involved in replication

Telomeres: special structures found at the end of eukaryotic chromosomes