Find a journal paper about PGLO plasmid in eukaryote trasfec

Find a journal paper about PGLO plasmid in eukaryote (trasfection process)

Solution

Answer:

pGLO plasmid is a cloning vector containing green fluorescent protein (GFP) and ampicillin resistance genes as the reporter genes. Other genes which are included in the vector are:

1. araC - Promoter DNA that regulates the expression of GFP

2. bla - Encodes beta-lactamase for clone selection

3. ori - Origin of replication

It also contains a multiple cloning site (MCS) for insertion of foreign gene.

It does not have any origin of replication for propagation in eukaryotic cells. So, these vectors are used solely in bacterial systems.

(No articles regarding transfection of pGLO in eukaryotes are present in the literature)