You are provided with a chinese hamster ovary cell line CHO2

You are provided with a chinese hamster ovary cell line CHO25 which expresses cell surface receptors for hormone X.

Describe how you would determine non-specific binding, total binding and specific binding of hormone X to these CHO25 cells.

Solution

To determine specific and Non-specific binding of hormone, Fluorescent Ligand Binding Assay.

In this method, you need to choose a Ligand/Drug specific to the receptor, which can be easily detached from he receptors without causing any harm to the receptor.

To check for total binding or partial binding -