the picture may be blurry but it is the basis for 2 question

the picture may be blurry but it is the basis for 2 questions..

1.) Can you give 2 distinctly different reasons for this change in shape of the standard curve (i.e. what could have caused this in a \"real\" experiment.)

2.) If you generated the results used in creating the protein standard curve for an experiment you were doing, would you use this standard curve data (yes or no or maybe)? You MUST explain your answer based on your results of the standard curve that generated to answer the question.

Solution

1.This may due to the potential issues associated with the method and it may due to the differences between nominal concentrations V/s predicted concentrations.

2.It is a Maybe.You can see points to points connected in the graph.The straight line is the linear regression for the entire set of standard points.Most people consider linear regression for the entire set of standards, assuming that the overall relationship between concentration and absorbance is best described by a straight line. As is obvious from the graph, this linear regression does not provide a good basis for interpolating correct test sample concentrations relative to assay results for the standards.

But using a curvilinear regression,this data can be seen as the most accurate for interpolating the test sample too.