Is the accompanying figure DNA or RNAIs the arrow closest to
Is the accompanying figure DNA or RNA?_______________Is the arrow closest to the 5\' or 3\' end? _______Spleen diesterase is an enzyme that breaks the covalent bond that connects the phosphate to the 5\' carbon. Assume that the dinucleotide is digested with spleen diesterase. To which base and to which carbon on the sugar is the phosphate now attached, A or T? RNA; 3\' end; A, 3\' DNA; 5\' end; A, 5\' DNA; 3\' end; T, 5\' RNA; 3\' end; T, 3\' DNA; 3\' end; A, 3\'
Solution
The correct answer would be: DNA ; 3\'end ; A, 3\'
Reason:It is a DNA structure as T is present. In RNA, T is not present.The arrow would indicate 3\' OH end to which nucleotides are added.The phosphate will now be attached to A at the 3\' end as spleen diesterase results in exonucleolytic cleavage that occurs in the 5\'- to 3\'-direction producing nucleoside 3\'-phosphates.In the above structure, since A is present towards 5\'end, so phosphate will be added to it and to 3\' carbon.
