Homework SoursePCR which of the following is correct Polymerase Chain React
PCR: which of the following is correct?
Polymerase Chain Reaction is? :
A. Utilizes either an upstream or downstream primer, but not both simultaneously.
B. Requires a large amount of template DNA to be successful.
C. Utalize the DNA polymerase of a thermophilic bacterium.
D. Can only be used to amplify DNA if the sequence is precisely known.
PCR: which of the following is correct?
Polymerase Chain Reaction is? :
A. Utilizes either an upstream or downstream primer, but not both simultaneously.
B. Requires a large amount of template DNA to be successful.
C. Utalize the DNA polymerase of a thermophilic bacterium.
D. Can only be used to amplify DNA if the sequence is precisely known.
Polymerase Chain Reaction is? :
A. Utilizes either an upstream or downstream primer, but not both simultaneously.
B. Requires a large amount of template DNA to be successful.
C. Utalize the DNA polymerase of a thermophilic bacterium.
D. Can only be used to amplify DNA if the sequence is precisely known.
Polymerase Chain Reaction is? :
A. Utilizes either an upstream or downstream primer, but not both simultaneously.
B. Requires a large amount of template DNA to be successful.
C. Utalize the DNA polymerase of a thermophilic bacterium.
D. Can only be used to amplify DNA if the sequence is precisely known.
Polymerase Chain Reaction is? :
A. Utilizes either an upstream or downstream primer, but not both simultaneously.
B. Requires a large amount of template DNA to be successful.
C. Utalize the DNA polymerase of a thermophilic bacterium.
D. Can only be used to amplify DNA if the sequence is precisely known.
Solution
Answer: (C) Utilize the DNA polymerase of a thermophilic bacterium.
PCR is short for polymerase chain reaction and is a method we can use to clone sequences of DNA. We often want to clone these sequences a lot, on the order of a billion copies of a single sequence.
The most important enzyme in a PCR reaction is called taq polymerase. Every cell that has DNA has its own polymerase that takes care of replication of DNA. PCR uses a polymerase from a species of bacteria, Thermus aquaticus, which normally lives in hot springs.
