Your microscope may have all or most of the features describ

Your microscope may have all or most of the features described below, and illustrated in figure 2.3 in your textbook. By studying this figure and reading your textbook, label the compound microscope in figure LR1.1 on page 10. Locate the indicated parts of your microscope and answer the following questions. a. What is the magnification stamped on the housing of the oculars on your microscope?_______ b. What are the magnifications of each of the objectives on your microscope?_______ c. Calculate the total magnification for each ocular/objective combination on your microscope. ____________ d. List the magnification and numerical aperture for each objective on your microscope. With some compound microscopes, loosening a lock screw allows you to rotate the body tube 180 degree. What is the advantage of being able to rotate the body tube?___________ f. Note the horizontal and vertical scales on the mechanical stage. What is the function of these scales? ____________ g. Where is the diaphragm on your microscope located? How can you regulate the diaphragm? _________ h. Locate the substage condenser on your microscope. What is its function, and how can it be regulated? i. Can the light intensity of your microscope be regulated? Explain._______

Solution

Answer

a) 5X, 10X or 15X. It can be changed for lower or higher magnification. Normally10X is used

b) 10X, 40X or 45X and 100X

c) Magnification = X value of the eye piece x X value of the objective.

ie 5X eye piece and 40X eye piece 5x40 = 200

for10X eye piece 10x 40 = 400

d) skipped

e) Body tube is rotated to adjust the level of position according to our comfort and to focus the area

f) Used to bring the desired area of the slide for observation.

g) Diaphragm is located below the stage of the microscope. It can be regulated by rotating

h) Function of condenser is to converge the light rays to the objective through the iris and the opening on the stage. Size of the aperture can be increased or decreased by using a pin, so that light rays can be regulated

i) Diaphragm helps in regulating the aperture size. Aperture size can be changed by using a needle and it controls the light passes through the slide.