THe blue rectangles represent the DNA wells that DNA was loa

THe blue rectangles represent the DNA wells that DNA was loaded into by each of the 10 groups of students. The white bands throughout the gel represent the PCR product, or DNA bands that light up under UV. The gray rectangles represent DNA smears.

Lab group number 5 unfortunately got no PCR product. What could their problem be AND how should they correct for this? Choose the most likely answer.

A)Their primers did not anneal to the DNA template. They should lower the annealing temperature.

B)The ammount of DNA template was too small. They should repeat the PCR but use more DNA template.

C)The primers annealed to the template, but at the wrong place. They should order new, more speciffic primers.

D)The template was not denatured sufficiently, so the primers could not get in and anneal. They need to increase the duration of the melting step.

Solution

B)The ammount of DNA template was too small. They should repeat the PCR but use more DNA template.