Homework SourseThe table below presents the results of different complete d
The table below presents the results of different complete digests of a linear DNA fragment. All sizes are in base pairs.
7. What is the total size of the undigested fragment?
8. Among the fragments obtained with the XbaI digest, which one has XbaI overhangs on both ends? Indicate the size of the fragment.
9. Digestion of the fragment indicated in question 8 with NruI would generate fragments of what sizes?
10. Among the fragments generated in the NcoI + NruI digest, which one could be ligated and cloned in a vector digested with NcoI + NruI? Indicate the size of the fragment.
11. What is the distance between the NcoI and NruI restriction sites?
12. What are the distances between the XbaI sites and the ends of the fragment?
PLEASE EXPLAIN HOW YOU GET THE ANSWERS!
| NcoI | 5023, 1295 |
| NruI | 4229, 2089 |
| XbaI | 3242, 2374, 702 |
| NcoI + NruI | 2934, 2089, 1295 |
| NcoI + XbaI | 3242, 1295, 1079, 702 |
| NruI + XbaI | 2374, 1855, 1387, 702 |
Solution
7.the undigested fragment is 6318bp (5023+1295)
8.3242(which comes in middle of both restriction sites)
9.1855,1387(total size 6318bp and 1855+1387=3242)
10.2934( vector digestion creates a proper site for this fragment ligation )
11.2934(this fragment comes in middle,there fore simple math give)
12.from one end,3944 &702
from another end 2374 & 5616 (Xbal sites come in order as 2374*3242*702 and from this structure it can easily understand)
